Introduction: Nowadays, patient safety is one of the basic principles in the world and medication Fulfillment of orders, an important part of treatment and care is also a major component of nursing performance. With knowledge of the nutritional status of the patient that is an important effect on the appropriate response to drug treatments, Can be prevented the risk of many adverse drug reactions including food-drug interactions that it also requires having knowledge of the case. This study aimed to comparison of pharmacology knowledge of food-drug interactions in ICU and general nurses was conducted. Materials and Methods: This is a descriptive and analytical study. The sample included all nurses in ICU and general ward that their number was 270. All ethical considerations in implementing this research in order to complete the questionnaire and acquiring necessary permits from the Council of the Faculty and maintaining the confidentiality of responses and statistical analysis were observance. Data gathering tool was a researcher made test consisted of two parts: The first part included demographic characteristics and, and the second part consisted of thirty multiple-choice questions to assess pharmacology knowledge of food-drug interactions. The responses were scored as 0 for incorrect and 1 for correct and the total score ranged from 0 to 30. A total number of 230 completed questionnaires were collected from participants and then analyzed by SPSS. 19 software. Descriptive and inferential statistics (independent t-test, variance analysis, and x2) were applied to the data. Results: the mean score for all the caring units was found to be 5.87±11.15 which was 6.09%±11.15 and 10.34±11.15 for general and intensive care units, respectively. There was no significant difference between the mean scores of different care units. Conclusion: it was revealed that the obtained mean scores were very low. Thus, it is necessary to take due actions such as developing and in-service training courses to promote nurses' pharmacological knowledge.