Objectives: Evaluating the effects of low-level laser (LLL) application on pain relief and abdominal scar formation in cesarean section (C-section) incision. Materials and Methods: The present prospective trial included 65 patients who referred to Amiralmomenin hospital of Semnan University of Medical Sciences for their first C-section. They were divided into intervention and control groups and LLL therapy was used in the intervention group after the randomization process. Then, scar formation and the level of pain in the incision site were evaluated after LLL imitation on 1, 3, 5, and 7 days of the post-partum period. As regards the scoring of the scar appearance and pain, 2 scoring methods were utilized, including Vancouver scar scale and visual analog scale. Results: A statistically significant difference was found between intervention and control groups in terms of pain score on days 5th and 10th of the post-partum period (P=0.000) and patients in the intervention group had a lower complaint of pain. However, no significant difference was detected between the 2 groups regarding Vancouver scar scale score which was used for scar formation in C-section incision. Conclusions: Overall, although pain relief is one of the most important complaints which patients suffer during the postsurgery period, other issues should be taken into account as well. By using this protocol, our study failed to prove the prophylactic role of LLL intervention in abdominal scar growth which is considered as another important point cosmetically. Accordingly, future studies might confirm the best protocol respecting preventing the scare formation with higher scale scores and reducing the pain.