Author(s)
Azizi M. Sadeghi Z. Bouzari S. Fasihi-Ramandi M.

Title: Mannosylated chitosan nanoparticles loaded with FliC antigen as a novel vaccine candidate against Brucella melitensis and Brucella abortus infection

Author(s): Azizi M.,Sadeghi Z.,Bouzari S.,Fasihi-Ramandi M.
منبع: Journal of Biotechnology : Volume 310, 2020 , Pages 89-96
نمایه شده در: Crossref WOS Scopus
شناسه دیجیتال: DOI:10.1016/j.jbiotec.2020.01.016
شناسه اختصاصی:
IRDOI
304-456-370-481
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لینک دانلود: [درخواست از نویسنده برای دریافت منبع] [دانلود از طریق گوگل اسکالر]
لینک ناشر: http://dx.doi.org/10.1016/j.jbiotec.2020.01.016
چکیده توضیحات بیشتر پیشنهاد ادامه پژوهش پیوست‌ها

Brucellosis is a worldwide bacterial zoonosis disease. Live attenuated Brucella vaccines have several drawbacks. Thus development of a safe and effective vaccine for brucellosis is a concern of many scientists. FliC protein contributes in virulence of Brucella; hence, it is a promising target for brucellosis vaccine. In this study, Mannosylated Chitosan Nanoparticles (MCN) loaded with FliC protein were synthesized as a targeted vaccine delivery system. The immunogenicity and protective efficacy of FliC and FliC-MCN against Brucella infection were evaluated in BALB/c mice. After cloning, expression and purification, FliC protein was loaded on MCN. The particle size, loading efficiency and in vitro release of the NPs were determined. Our investigation revealed that FliC and FliC-MCN could significantly increase specific IgG response (higher IgG2a titers). Besides, spleen cells from immunized mice produced high level of IFN-γ and IL-2 and low level IL-10 cytokines. Immunization with FliC and FliC-MCN conferred significant degree of protection against B. melitensis 16 M and B. abortus 544 infections. Overall these results indicate that FliC protein would be a novel potential antigen candidate for the development of a subunit vaccine against B. melitensis and B. abortus. Moreover, MCN could be used as an adjuvant and targeted vaccine delivery system. © 2020
Keywords
Degree of protection animal cell FliC Brucella infection dispersity nanocarrier zeta potential physical parameters MICE Brucella molecular cloning Proteins Antigens OUTER-MEMBRANE PROTEIN-31 recombinant flic antigen animal experiment protein expression immunoglobulin G1 infection prevention brucellosis Clone cells CONFERS PROTECTION Chitosan spleen cell mannosylated chitosan nanoparticle Brucella melitensis Mannosylated chitosan nanoparticle cytokine response drug delivery system loading efficiency Immunization nonhuman REVERSE VACCINOLOGY female chitosan nanoparticle Particle size Protective efficacy animal model gamma interferon protein purification Brucella abortus immunoglobulin G2a immunoglobulin M IMMUNE-RESPONSE DNA VACCINE cytokine production cross protection interleukin 2 controlled study humoral immunity Loading efficiency immunoglobulin G3 CELLS vaccination interleukin 10 in vitro study animal tissue Bagg albino mouse cellular immunity Nanoparticles drug efficacy drug release Brucella vaccine immunoglobulin G2b recombinant antigen Mannosylated chitosans nanopharmaceutics Article mouse particle size IMMUNIZATION priority journal Mammals immunoglobulin A DELIVERY Vaccines subunit vaccine Vaccine delivery system vaccine immunogenicity IMMUNOGENICITY Th1 cell Biosynthesis unclassified drug

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