The most important iron ore deposits of Iran, namely Chogart, Gol-e-Gohar, Chador Malu, are located in central Iran. The main iron bearing mineral phases of these deposits were known as magnetite, martite and hematite, which are finely intergrown with apatite and silicates. In the beneficiation plant of Chador Malu, the dephosphorization of high-intensity magnetic separation concentrate is carried out by floating the phosphorus gangue from iron oxide fines. The main problem during the reverse flotation of iron oxide fines is the stabilization of the P-content in the concentrate to an acceptable level of less than 0.045 wt%, and to minimize the loss of iron oxides in the flotation circuit. The main objective of this study was to identify the conditions under which the amount of the phosphorus of iron oxide fines can be reduced and stabilized to a level of less than 0.045 %. From the results of this study, it can be pointed out that particle size distribution, pulp pH and temperature as well as collector and depressant type and dosage are the key variables that need to be controlled in the dephosphorization process. Using a new collector, more than 82 % of iron oxide fines were recovered at optimal flotation conditions. The phosphorus content of flotation concentrate could be adjusted around 0.040 wt%.